Journal: Frontiers in Immunology
Article Title: Neoadjuvant Radiochemotherapy Significantly Alters the Phenotype of Plasmacytoid Dendritic Cells and 6-Sulfo LacNAc + Monocytes in Rectal Cancer
doi: 10.3389/fimmu.2019.00602
Figure Lengend Snippet: Rectal cancer-infiltrating pDCs can express CXCL10 and CCL4. (A,B) Immunofluorescence stainings were conducted to analyze the presence of CXCL10- or CCL4-expressing pDCs in 9 pre-nRCT and 18 post-nRCT tumor specimens of the matched cohort. As representative examples, images of single (A) CXCL10 and BDCA-2 or single (B) CCL4 and BDCA-2 stainings as well as merged images are shown. Original magnification was x200.
Article Snippet: For the two-color Opal staining, the BDCA-2 primary antibody was used together with the polyclonal goat anti-human CXCL10, CCL4, or CCL5 primary antibody (1:100, all from R&D Systems, low pH retrieval) and combined with the TSA fluorophores 650 (1:100) and 570 (1:100), respectively.
Techniques: Immunofluorescence, Expressing